PULSE BIOSCIENCES INC

Ticker: PLSE CUSIP: 74587B101 Class: Common Stock

TWO SIGMA INVESTMENTS, LP's Holding History (CIK: 0001179392)

Note: Option positions (PUT/CALL) are excluded.

Q1 2022

Shares 59,636 Value ($000) $290 Avg Close $8.18 Range $4.14 - $15.38

Q3 2021

Shares 21,074 Value ($000) $455 Avg Close $21.51 Range $16.66 - $29.16

Q2 2021

Shares 71,130 Value ($000) $1,167 Avg Close $19.03 Range $14.59 - $24.20

Q1 2021

Shares 64,201 Value ($000) $1,520 Avg Close $29.89 Range $20.76 - $45.82

Period	Shares	Value ($000)	Average Close	Price Range
Q1 2022	59,636	$290	$8.18	$4.14 - $15.38
Q3 2021	21,074	$455	$21.51	$16.66 - $29.16
Q2 2021	71,130	$1,167	$19.03	$14.59 - $24.20
Q1 2021	64,201	$1,520	$29.89	$20.76 - $45.82